20 results found for: “taq_polymerase”.

Request time (Page generated in 0.354 seconds.)

Taq polymerase

Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally...

Last Update: 2024-04-11T04:02:52Z Word Count : 2604 Synonim Taq polymerase

Polymerase chain reaction

heat-stable DNA polymerase, such as Taq polymerase, an enzyme originally isolated from the thermophilic bacterium Thermus aquaticus. If the polymerase used was...

Last Update: 2024-03-23T04:17:02Z Word Count : 11116 Synonim Polymerase chain reaction

DNA polymerase

and nature. Taq polymerase is a heat-stable enzyme of this family that lacks proofreading ability. DNA polymerase II is a family B polymerase encoded by...

Last Update: 2024-03-29T22:05:04Z Word Count : 7054 Synonim DNA polymerase

TaqMan

the TaqMan probe significantly increases the specificity of the detection. TaqMan probes were named after the videogame Pac-Man (Taq Polymerase + PacMan...

Last Update: 2023-12-23T02:15:54Z Word Count : 684 Synonim TaqMan

Hot start PCR

such as inactivating or inhibiting the binding of Taq polymerase and late addition of Taq polymerase, to increase product yield as well as provide a higher...

Last Update: 2023-04-30T14:49:44Z Word Count : 2750 Synonim Hot start PCR

Polymerase

replication. A DNA polymerase from the thermophilic bacterium, Thermus aquaticus (Taq) (PDB 1BGX, EC 2.7.7.7) is used in the polymerase chain reaction, an...

Last Update: 2023-10-24T14:35:27Z Word Count : 673 Synonim Polymerase

Pfu DNA polymerase

and proofreading properties compared with Taq DNA polymerase. Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading...

Last Update: 2023-11-04T10:08:14Z Word Count : 446 Synonim Pfu DNA polymerase

Reverse transcription polymerase chain reaction

one-step RT-PCR kit with a mix of reverse transcriptase, Taq DNA polymerase, and a proofreading polymerase is selected and all necessary materials and equipment...

Last Update: 2024-03-23T16:08:36Z Word Count : 5537 Synonim Reverse transcription polymerase chain reaction

Thermus aquaticus

heat-resistant enzyme Taq DNA polymerase, one of the most important enzymes in molecular biology because of its use in the polymerase chain reaction (PCR)...

Last Update: 2024-04-13T18:12:58Z Word Count : 1792 Synonim Thermus aquaticus

History of polymerase chain reaction

December 22, 1989, the journal Science awarded Taq Polymerase (and PCR) its first "Molecule of the Year". The 'Taq PCR' paper became for several years the most...

Last Update: 2024-02-08T10:26:56Z Word Count : 3101 Synonim History of polymerase chain reaction

Taq

Taq or TAQ may refer to: Taq, Iran, a village in Semnan Province, Iran Taq polymerase, a heat-stable enzyme used in polymerase chain reaction, or Thermus...

Last Update: 2023-07-21T04:08:50Z Word Count : 106 Synonim Taq

TOPO cloning

cloned into specific vectors without the requirement for DNA ligases. Taq polymerase has a nontemplate-dependent terminal transferase activity that adds...

Last Update: 2023-01-26T19:49:06Z Word Count : 568 Synonim TOPO cloning

Thermococcus litoralis

produce an alternative DNA polymerase to the commonly used Taq polymerase. The T. litoralis polymerase, dubbed the vent polymerase, has been shown to have...

Last Update: 2023-03-21T23:48:35Z Word Count : 1023 Synonim Thermococcus litoralis

Real-time polymerase chain reaction

breakdown of the probe by the 5' to 3' exonuclease activity of the Taq polymerase breaks the reporter-quencher proximity and thus allows unquenched emission...

Last Update: 2023-12-03T16:46:37Z Word Count : 5338 Synonim Real-time polymerase chain reaction

DNA polymerase I

first protocols of polymerase chain reaction (PCR) amplification until Thermus aquaticus, the source of a heat-tolerant Taq Polymerase I, was discovered...

Last Update: 2023-12-10T13:34:32Z Word Count : 1871 Synonim DNA polymerase I

Master mix (PCR)

(required as a substrate for the building of new DNA strands), MgCl2, Taq polymerase (an enzyme required to building new DNA strands), a pH buffer and come...

Last Update: 2024-02-13T22:36:29Z Word Count : 270 Synonim Master mix (PCR)

Polymerase chain reaction optimization

fidelity amplification compared with Taq. Magnesium is required as a co-factor for thermostable DNA polymerase. Taq polymerase is a magnesium-dependent enzyme...

Last Update: 2023-12-13T22:39:14Z Word Count : 2029 Synonim Polymerase chain reaction optimization

Kary Mullis

Saiki started to use Thermophilus aquaticus (Taq) DNA polymerase to amplify segments of DNA. The Taq polymerase was heat resistant and needed to be added...

Last Update: 2024-03-06T10:22:07Z Word Count : 4845 Synonim Kary Mullis

Klenow fragment

segments of DNA in the polymerase chain reaction (PCR) process, before being replaced by thermostable enzymes such as Taq polymerase. Just as the 5' → 3'...

Last Update: 2024-03-27T05:39:16Z Word Count : 472 Synonim Klenow fragment

Thermophile

Some of these enzymes are used in molecular biology, for example the Taq polymerase used in PCR. "Thermophile" is derived from the Greek: θερμότητα (thermotita)...

Last Update: 2024-03-31T21:24:31Z Word Count : 1256 Synonim Thermophile

Main result

Taq polymerase

Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by Chien et al. in 1976. Its name is often abbreviated to Taq or Taq pol. It is frequently used in the polymerase chain reaction (PCR), a method for greatly amplifying the quantity of short segments of DNA. T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR. Therefore, it replaced the DNA polymerase from E. coli originally used in PCR.