20 results found for: “proteinase_k”.

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Proteinase K

molecular biology, Proteinase K (EC 3.4.21.64, protease K, endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, Tritirachium...

Last Update: 2023-11-07T16:42:20Z Word Count : 1034 Synonim Proteinase K

Prion

to relate to PrP structure or function. PrPC is readily digested by proteinase K and can be liberated from the cell surface in vitro by the enzyme phosphoinositide...

Last Update: 2024-03-26T17:17:13Z Word Count : 10636 Synonim Prion

Protein K (porin)

Proteinase K has a catalytic triad of Ser 224, His 69, and Asp 39. Two peptide chains recognize substrates, 99-104 and 132–136. There is also a cysteine...

Last Update: 2023-01-26T01:56:53Z Word Count : 269 Synonim Protein K (porin)

Staphylococcus haemolyticus

carbohydrates, proteins, and extracellular DNA. Detachment assays with NaIO4, proteinase K, or DNase result in 38%, 98%, and 100% detachment, respectively. The...

Last Update: 2023-11-28T19:27:54Z Word Count : 2402 Synonim Staphylococcus haemolyticus

Proteolysis

activity or removal of unwanted proteins. For example, proteinase K, a broad-spectrum proteinase stable in urea and SDS, is often used in the preparation...

Last Update: 2023-11-07T15:44:57Z Word Count : 4225 Synonim Proteolysis

Cross-linking immunoprecipitation

separated from free RNA using gel electrophoresis and membrane transfer. Proteinase K digestion is then performed in order to remove protein from the crosslinked...

Last Update: 2024-02-22T11:50:47Z Word Count : 4888 Synonim Cross-linking immunoprecipitation

Complementary DNA

RNA unusable. To remove DNA and proteins, enzymes such as DNase and Proteinase K are used for degradation. Importantly, RNA integrity is maintained by...

Last Update: 2024-02-18T19:34:12Z Word Count : 2275 Synonim Complementary DNA

DRIP-seq

termination. First, genomic DNA (gDNA) is extracted from cells of interest by proteinase K treatment followed by phenol-chloroform extraction and ethanol precipitation...

Last Update: 2023-12-03T20:31:41Z Word Count : 1997 Synonim DRIP-seq

Aspergillopepsin I

Aspergillus acid proteinase, Aspergillus aspartic proteinase, Aspergillus awamori acid proteinase, Aspergillus carboxyl proteinase, carboxyl proteinase, Aspergillus...

Last Update: 2023-08-26T13:15:44Z Word Count : 499 Synonim Aspergillopepsin I

In situ hybridization

fluorescence or brightfield microscope. permeabilization of cells with proteinase K to open cell membranes (around 25 minutes, not needed for tissue sections...

Last Update: 2024-03-19T17:22:13Z Word Count : 1318 Synonim In situ hybridization

DNA extraction

used to break open cells and release the DNA. Protease enzymes, such as Proteinase K, which are used to digest proteins that may be binding to the DNA. Phenol...

Last Update: 2024-03-09T15:17:10Z Word Count : 3168 Synonim DNA extraction

Oryzin

alkaline proteinase, Aspergillus melleus semi-alkaline proteinase, Aspergillus oryzae alkaline proteinase, Aspergillus parasiticus alkaline proteinase, Aspergillus...

Last Update: 2023-08-26T15:15:41Z Word Count : 308 Synonim Oryzin

Creutzfeldt–Jakob disease

detection of prion-infected material in whole human blood without the use of proteinase K" (PDF). Transfusion (Submitted manuscript). 50 (12): 2619–27. doi:10...

Last Update: 2024-03-16T15:57:05Z Word Count : 7417 Synonim Creutzfeldt–Jakob disease

Protease

A protease (also called a peptidase, proteinase, or proteolytic enzyme) is an enzyme that catalyzes proteolysis, breaking down proteins into smaller polypeptides...

Last Update: 2023-12-15T03:08:43Z Word Count : 2749 Synonim Protease

Polylactic acid

can also degrade PLA. Enzymes such as pronase and most effectively proteinase K from Tritirachium album degrade PLA. Four possible end-of-life scenarios...

Last Update: 2024-02-25T14:45:46Z Word Count : 4313 Synonim Polylactic acid

Pronase

improve image quality in gastroscopy by thinning the mucus in advance. Proteinase K Teh, Jun-Liang; Shabbir, Asim; Yuen, Soon; So, Jimmy Bok-Yan (2020)....

Last Update: 2022-06-10T08:55:19Z Word Count : 129 Synonim Pronase

Antigen retrieval

proteolytic induced epitope retrieval (PIER). In PIER, enzymes including Proteinase K, Trypsin, and Pepsin have been used to restore antibody binding to its...

Last Update: 2023-10-26T18:15:16Z Word Count : 419 Synonim Antigen retrieval

Caricain

papaya peptidase II, papaya proteinase, papaya proteinase III, papaya proteinase 3, proteinase omega, papaya proteinase A, chymopapain S, Pp) is an enzyme...

Last Update: 2023-11-08T14:15:20Z Word Count : 2014 Synonim Caricain

Environmental DNA

biocon.2014.11.019. Tsuji, Satsuki (2016). "Effects of water pH and proteinase K treatment on the yield of environmental DNA from water samples". Limnology...

Last Update: 2024-03-20T03:06:02Z Word Count : 13420 Synonim Environmental DNA

Kexin

gene KEX2 dibasic proteinase, Kex 2p proteinase, Kex2 endopeptidase, Kex2 endoprotease, Kex2 endoproteinase, Kex2 protease, proteinase Kex2p, Kex2-like...

Last Update: 2023-09-01T22:17:23Z Word Count : 504 Synonim Kexin

Main result

Proteinase K

In molecular biology, Proteinase K (EC 3.4.21.64, protease K, endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, Tritirachium album proteinase K) is a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Parengyodontium album (formerly Engyodontium album or Tritirachium album). Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8 (subtilisin). The molecular weight of Proteinase K is 28,900 daltons (28.9 kDa).


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