20 results found for: “proteinase_k”.

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Proteinase K

molecular biology Proteinase K (EC 3.4.21.64, protease K, endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, Tritirachium...

Last Update: 2021-02-05T04:00:21Z Word Count : 1065 Synonim Proteinase K

Prion

to relate to PrP structure or function. PrPC is readily digested by proteinase K and can be liberated from the cell surface in vitro by the enzyme phosphoinositide...

Last Update: 2021-05-11T09:38:55Z Word Count : 8678 Synonim Prion

DRIP-seq

termination. First, genomic DNA (gDNA) is extracted from cells of interest by proteinase K treatment followed by phenol-chloroform extraction and ethanol precipitation...

Last Update: 2021-02-12T02:41:48Z Word Count : 1972 Synonim DRIP-seq

Proteolysis

activity or removal of unwanted proteins. For example, proteinase K, a broad-spectrum proteinase stable in urea and SDS, is often used in the preparation...

Last Update: 2021-05-06T23:41:14Z Word Count : 4218 Synonim Proteolysis

Complementary DNA

RNA unusable. To remove DNA and proteins, enzymes such as DNase and Proteinase K are used for degradation. Importantly, RNA integrity is maintained by...

Last Update: 2021-04-26T21:58:45Z Word Count : 2092 Synonim Complementary DNA

In situ hybridization

fluorescence or brightfield microscope. permeabilization of cells with proteinase K to open cell membranes (around 25 minutes, not needed for tissue sections...

Last Update: 2021-03-09T11:36:41Z Word Count : 1307 Synonim In situ hybridization

Creutzfeldt–Jakob disease

detection of prion-infected material in whole human blood without the use of proteinase K" (PDF). Transfusion (Submitted manuscript). 50 (12): 2619–27. doi:10...

Last Update: 2021-05-11T18:14:16Z Word Count : 6789 Synonim Creutzfeldt–Jakob disease

Staphylococcus haemolyticus

carbohydrates, proteins, and extracellular DNA. Detachment assays with NaIO4, proteinase K, or DNase result in 38%, 98%, and 100% detachment, respectively. The...

Last Update: 2021-04-24T16:05:16Z Word Count : 2393 Synonim Staphylococcus haemolyticus

Pronase

the inactivation gate of Na+ voltage gated ion channels in neurons. Proteinase K Pronase at the US National Library of Medicine Medical Subject Headings...

Last Update: 2020-06-04T20:45:52Z Word Count : 72 Synonim Pronase

Polylactic acid

can also degrade PLA. Enzymes such as pronase and most effectively proteinase K from Tritirachium album degrade PLA. Four possible end-of-life scenarios...

Last Update: 2021-05-12T22:25:01Z Word Count : 3745 Synonim Polylactic acid

Subtilopeptidase A

Subtilopeptidase A may refer to: Subtilisin, EC 3.4.21.62 Oryzin, EC 3.4.21.63 Proteinase K, EC 3.4.21.64 Thermomycolin, EC 3.4.21.65 Thermitase, EC 3.4.21.66 Endopeptidase...

Last Update: 2021-05-03T21:43:42Z Word Count : 76 Synonim Subtilopeptidase A

3C-like protease

coronavirus 3C-like proteinase". The Journal of Biological Chemistry. 279 (3): 1637–42. doi:10.1074/jbc.m310875200. PMID 14561748. Akaji K, Konno H, Onozuka...

Last Update: 2021-05-09T07:41:24Z Word Count : 1646 Synonim 3C-like protease

Antigen retrieval

proteolytic induced epitope retrieval (PIER). In PIER, enzymes including Proteinase K, Trypsin, and Pepsin have been used to restore antibody binding to its...

Last Update: 2021-05-03T01:54:13Z Word Count : 392 Synonim Antigen retrieval

Chromatin immunoprecipitation

protein–DNA cross-link is reversed and proteins are removed by digestion with proteinase K. An epitope-tagged version of the protein of interest, or in vivo biotinylation...

Last Update: 2021-01-21T23:48:39Z Word Count : 2531 Synonim Chromatin immunoprecipitation

Cross-linking immunoprecipitation

separated from free RNA using gel electrophoresis and membrane transfer. Proteinase K digestion is then performed in order to remove protein from the RNA-protein...

Last Update: 2021-04-15T17:38:56Z Word Count : 3428 Synonim Cross-linking immunoprecipitation

Tryptase

(EC 3.4.21.59, ) is the most abundant secretory granule-derived serine proteinase contained in mast cells and has been used as a marker for mast cell activation...

Last Update: 2021-05-06T22:14:58Z Word Count : 788 Synonim Tryptase

VPg

(a protease) cleaves VPg from membrane-bound 3AB. Studies that used proteinase K to cleave VPg from the viral genome discovered that calicivirus vesicular...

Last Update: 2020-12-27T13:48:31Z Word Count : 730 Synonim VPg

Sedolisin

PMID 22016395. Oda K, Sugitani M, Fukuhara K, Murao S (March 1987). "Purification and properties of a pepstatin-insensitive carboxyl proteinase from a gram-negative...

Last Update: 2021-05-05T21:40:29Z Word Count : 821 Synonim Sedolisin

PCSK9

a novel proteinase related to proteinase K". Arch. Biochem. Biophys. 420 (1): 55–67. doi:10.1016/j.abb.2003.09.011. PMID 14622975. Ouguerram K, Chetiveaux...

Last Update: 2021-05-05T19:32:38Z Word Count : 7066 Synonim PCSK9

Proteinase 3

Proteinase 3, also known as PRTN3, is an enzyme that in humans is encoded by the PRTN3 gene. PRTN3 is a serine protease enzyme expressed mainly in neutrophil...

Last Update: 2021-05-07T23:25:14Z Word Count : 893 Synonim Proteinase 3

Main result

Proteinase K

In molecular biology Proteinase K (EC 3.4.21.64, protease K, endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, Tritirachium album proteinase K) is a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album). Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8 (subtilisin). The molecular weight of Proteinase K is 28,900 daltons (28.9 kDa).


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