Proteinase K

Proteinase K
Structure of Proteinase K.[1]
Identifiers
EC no.3.4.21.64
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
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PMCarticles
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NCBIproteins
Proteinase K
Identifiers
OrganismEngyodontium album
SymbolPROK
UniProtP06873
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StructuresSwiss-model
DomainsInterPro

In molecular biology, Proteinase K (EC 3.4.21.64, protease K, endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, Tritirachium album proteinase K) is a broad-spectrum serine protease.[2][3][4] The enzyme was discovered in 1974 in extracts of the fungus Parengyodontium album (formerly Engyodontium album or Tritirachium album).[5] Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8 (subtilisin). The molecular weight of Proteinase K is 28,900 daltons (28.9 kDa).

  1. ^ Betzel C, Singh TP, Visanji M, Peters K, Fittkau S, Saenger W, Wilson KS (July 1993). "Structure of the complex of proteinase K with a substrate analogue hexapeptide inhibitor at 2.2-A resolution". J. Biol. Chem. 268 (21): 15854–8. doi:10.1016/S0021-9258(18)82332-8. PMID 8340410.
  2. ^ Morihara K, Tsuzuki H (1975). "Specificity of proteinase K from Tritirachium album Limber for synthetic peptides". Agric. Biol. Chem. 39 (7): 1489–1492. doi:10.1271/bbb1961.39.1489.
  3. ^ Kraus E, Kiltz HH, Femfert UF (February 1976). "The specificity of proteinase K against oxidized insulin B chain". Hoppe-Seyler's Z. Physiol. Chem. 357 (2): 233–7. PMID 943367.
  4. ^ Jany KD, Lederer G, Mayer B (1986). "Amino acid sequence of proteinase K from the mold Tritirachium album Limber". FEBS Lett. 199 (2): 139–144. doi:10.1016/0014-5793(86)80467-7. S2CID 85577597.
  5. ^ Ebeling W, Hennrich N, Klockow M, Metz H, Orth HD, Lang H (August 1974). "Proteinase K from Tritirachium album Limber". Eur. J. Biochem. 47 (1): 91–7. doi:10.1111/j.1432-1033.1974.tb03671.x. PMID 4373242.