Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidine. BrdU is commonly used to study cell proliferation in living tissues and has been studied as a radiosensitizer and diagnostic tool in people with cancer.
BrdU (bromodeoxyuridine) is a thymidine analog that can be incorporated into DNA during cell division. Learn how to label, stain and detect BrdU with anti-BrdU antibodies and other methods for cell cycle analysis.
Learn how to label and detect BrdU (Bromodeoxyuridine), an analog of thymidine used to identify proliferating cells. Find out how to perform BrdU labeling in vitro or in vivo, and how to perform DNA hydrolysis and co-staining with anti-BrdU antibodies.
Bromodeoxyuridine (BrdU) is a thymidine analogue that can be used to label and detect cell proliferation, survival, and differentiation in various tissues and organisms. Learn how BrdU is incorporated into DNA, detected by immunohistochemistry, and applied to study brain regeneration, synaptogenesis, and cell cycle.
Effects of Bromodeoxyuridine Exposure on the Cerebellar External Granular Layer Neuroblasts The EGL is a transient proliferative matrix of growing cerebellar folia located beneath the piamater. In most of the vertebrates, this structure increases in thickness during an initial period of time as a result of proliferative activity of its ...
Bromodeoxyuridine (BrdUrd), a thymidine analogue incorporated into DNA, can be quantified by fluorescent or chromophoric quenching of dyes bound to DNA or with antibodies to BrdUrd. These technologies have been used since the 1970s as tools for measuring DNA synthesis in isolated chromosomes and in cells and tissues. This paper is Part I of a three-part comprehensive review of the literature ...
As markers of DNA synthesis, [3 H]thymidine ([3 H]dT) and the later developed analog bromodeoxyuridine (BrdU) have revolutionized our ability to identify dividing cells and follow their fate in various tissues, including the nervous system.However, the effect of the incorporation of these molecules into DNA on cell proliferation, migration, differentiation, and function is not fully understood.
Abstract. Incorporation of bromodeoxyuridine (BrdU) during DNA replication is frequently used for cell cycle analysis. The flow cytometric BrdU/Hoechst quenching technique is conducive to high‐resolution assessment of cell cycle kinetics, but requires continuous BrdU treatment, which may have cytostatic or cytotoxic effects.
Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU immunohistochemistry has been instrumental for the study of the development of the nervous system, and to confirm that neurogenesis occurs in ...
Learn about the properties, preparation, and uses of 5-bromo-2-deoxyuridine (BrdU), a brominated analog of thymidine that is incorporated into cell DNA. This product is for R&D use only and should be stored at -20 C.
Bromodeoxyuridine (BrdU) is a thymidine analog to which monoclonal antibodies have been developed. This methodology allows detection of endothelial cells in the S phase at the light microscopic level but, unlike autoradiography, there is no 3- to 4-week exposure period. Labeling indices can be calculated immediately after the immunocytochemical ...
Using this method, we detect 5-bromodeoxyuridine (BrdU) incorporated by Saccharomyces cerevisiae to reveal, at a genomic scale and on single molecules, the DNA sequences replicated during a pulse ...
Further studies demonstrate that BrdU promotes full-chemical induction of mouse iPSCs using several chemical cocktails, with the minimal combination being BrdU, CHIR99021, Repsox, and Forskolin ...
Bromodeoxyuridine (BrdU) is a synthetic thymidine analog that gets incorporated into a cell's DNA when the cell is dividing (during the S-phase of the cell). BrdU is commonly used in the detection of proliferating cells in living tissues or as a cell cycle marker. It is administered to animals either shortly before euthanasia or as a chronic ...
As markers of DNA synthesis, [3H]thymidine ([3H]dT) and the later developed analog bromodeoxyuridine (BrdU) have revolutionized our ability to identify dividing cells and follow their fate in various tissues, including the nervous system. However, the effect of the incorporation of these molecules into DNA on cell proliferation, migration, differentiation, and function is not fully understood ...
May 2011. What is Bromodeoxyuridine? Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is an odourless white powder, a synthetic nucleoside and an analogue of thymidine.BrdU is commonly used in the detection of proliferating cells in living tissues and works by substituting for thymidine during DNA replication thereby incorporating itself into the newly synthesized DNA.
5-bromodeoxyuridine is a mutagen (causes mutations), a cytotoxin, a teratogen and a weak carcinogen. The primary harmful effects are genetic mutation, anemia, reproductive disorders (fetal death or abnormality), cataracts, and skin irritation. It can cause respiratory tract irritation if inhaled, skin irritation if it contacts the skin and eye ...
Bromodeoxyuridine has a long history as a potential anticancer drug, and it is known that at high doses and in combination with secondary stressors, such as ionizing radiation, BrdU can have lethal consequences for incorporating cells. The present findings are surprising in that our BrdU regimen is exceedingly mild, even by current experimental ...
BrdU is a thymidine analog used in cell proliferation studies. Learn how to label cells in vitro or in vivo with BrdU and detect BrdU incorporation by anti-BrdU antibodies.
Bromodeoxyuridine (BrdUrd), a thymidine analogue incorporated into DNA, can be quantified by fluorescent or chromophoric quenching of dyes bound to DNA or with antibodies to BrdUrd. These technologies have been used since the 1970s as tools for measuring DNA synthesis in isolated chromosomes and in …
5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation. 5-Bromo-2′-deoxyuridine has been used:
The objective of this work is to evaluate the influence of pomegranate peel extract (PPE) in the behavior of breast cell lines (epithelial and tumor type) and related oxidative metabolism. Fruit-based functional foods have been the target of increasing scientific research for their physiological and pathophysiological properties. Pomegranate (Punica granatum) is a suitable example with both ...
bro· mo· de· oxy· ur· i· dine ˌbrō-mō-ˌdē-ˌäk-sē-ˈyu̇r-ə-ˌdēn, -dən. variants or 5-bromodeoxyuridine. ˈfīv-. : a mutagenic analog C9H11O5NBr of thymidine that induces chromosomal breakage especially in heterochromatic regions and has been used to selectively destroy actively dividing cellsabbreviation BUdR.
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Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidineproliferative index include mitotic indexing, thymidine-labeling index, bromodeoxyuridine assay, the determination of fraction of cells in various phases ofKinase. "Induction of Endogenous Virus and of Thymidline Kinase by Bromodeoxyuridine in Cell Cultures Transformed by Friend Virus" (PDF). Archived (PDF)lack one of these genes. Thus, a cell lacking TK is resistant to bromodeoxyuridine (BrdU) and a cell lacking HGPRT is resistant to 6-thioguanine (6-TG)proliferation can be measured this way in lymphoproliferative disorders. Bromodeoxyuridine (BrdU) is another thymidine analog that is often used for the detectionandrogens and a lack of external male genitalia. Neural injections of Bromodeoxyuridine (BrdU) were applied to males of both groups to test for neurogenesisstaining.[citation needed] Hoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst 33342drugs in animals. Examples of these studies include injection of bromodeoxyuridine for proliferation tracing, Apelin-13 for cerebral ischemia, and α-interferon Newmark, Phillip A.; Sánchez Alvarado, Alejandro (2000-04-15). "Bromodeoxyuridine Specifically Labels the Regenerative Stem Cells of Planarians". Developmentallevel can be caused by: Chemicals Hydroxylamine Base analogs (e.g., Bromodeoxyuridine (BrdU)) Alkylating agents (e.g., N-ethyl-N-nitrosourea (ENU). Thesedyed. The Giemsa staining was able to stain due to the presence of bromodeoxyuridine analogous base which was introduced to the desired chromatid. Thedoing an experiment in which the cells were treated in vivo with bromodeoxyuridine. In other experiments in mouse, it has been shown that the lifespanresistant to estrogens and antiestrogens, possibly due to inhibition by 5-bromodeoxyuridine and sodium butyrate. Yu, S; et al. (6 May 2017). "The T47D cell linecan also be decapped. 5' untranslated region (5'-UTR) 5-bromodeoxyuridine See bromodeoxyuridine. 5'-end One of two ends of a single linear strand of DNAtriarylmethane 76-60-8 Bromocresol purple BCP triarylmethane 115-40-2 Bromodeoxyuridine BDU 59-14-3 Bromophenol blue BPB Albutest triarylmethane 115-39-9azide–alkyne cycloaddition (CuAAC) click chemistry. Unlike the commonly used bromodeoxyuridine (BrdU), EdU detection requires no heat or acid treatment. EdU incorporatedStrand-seq requires dividing cells for strand-specific labelling using bromodeoxyuridine (BrdU), and the method does not detect variants smaller than 200kb1127. PMID 13860748. S2CID 1606140. Kuhn HG, Cooper-Kuhn CM (2007). "Bromodeoxyuridine and the detection of neurogenesis". Curr Pharmaceutical Biotechnolovercomes the limitations of traditional approaches (e.g. the classic Bromodeoxyuridine (BrdU) labeling technique has been used to identify slow-cycling cellsexamples, see ), and nonfolate-sensitive groups, which are induced by bromodeoxyuridine (BrdU) or distamycin A, an antibiotic that preferentially binds toClaire; Jackie Hunter, A.; Thorndyke, Michael C. (1998). "Patterns of bromodeoxyuridine incorporation and neuropeptide immunoreactivity during arm regenerationparental DNA strands with a DNA label such as tritiated thymidine or bromodeoxyuridine (BrdU). These types of DNA labels will incorporate into the newlyeither in vivo or in vitro. During S-phase cells are treated with bromodeoxyuridine (BrdU) which is then incorporated into their nascent DNA, acting as(November 2013). "Identification of active oxalotrophic bacteria by Bromodeoxyuridine DNA labeling in a microcosm soil experiments". FEMS Microbiology Lettersantibodies against BrdU followed by flow cytometry. 5-Bromouracil Bromodeoxyuridine 5-bromouridine in Linstrom, Peter J.; Mallard, William G. (eds.);Merrick CJ (December 2015). "Transfection with thymidine kinase permits bromodeoxyuridine labelling of DNA replication in the human malaria parasite PlasmodiumVirus: High-Frequency Activation in vitro by 5-Iododeoxyuridine and 5-Bromodeoxyuridine". Science. 174 (4005): 155–156. Bibcode:1971Sci...174..155L. doi:10new method of non-radioactive labelling, based on incorporation of bromodeoxyuridine into single-stranded probes. The method was used to localise satellitein the developing telencephalon: pulse-labeling chick embryos with bromodeoxyuridine". Journal of Neuroscience. 20 (21): 8021–8030. doi:10.1523/jneuroscitypically involve incorporating modified nucleotides (such as BrdU, bromodeoxyuridine) into the nascent DNA, then fluorescently detecting it. As replicationhatchlings and adults in the marine platyhelminth Macrostomum sp.: A bromodeoxyuridine analysis". Developmental Biology. 226 (2): 231–241. doi:10.1006/dbiohemoglobin synthesis, and the control of cell division. The effects of bromodeoxyuridine on cell differentiation (conversion of a primitive cell into a moreare located in the renal papilla. Using stain-retaining assay (with bromodeoxyuridine, or BrdU), a low-cycling cell population was found in the papillaryAcad. Sci. USA, 56:1151-54. 1967 With W. H. Taliaferro. Effect of 5-bromodeoxyuridine on the hemolysin response in rabbits. Proc. Soc. Exp. Biol. Med.,GM1 Bcl-2 Beta-galactosidase (β-gal) of ROSA26 mice beta1-integrin bromodeoxyuridine c-kit (CD117) c-Met C1qR(p) END (CD105) PROM1 (CD133) ALCAM (CD166)deoxyguanosine MeSH D13.570.230.430 – deoxyuridine MeSH D13.570.230.430.196 – bromodeoxyuridine MeSH D13.570.230.430.432 – floxuridine MeSH D13.570.230.430.609 –assay from Biovica International uses another thymidine analogue, bromodeoxyuridine, as substrate to the enzyme. The product of the reaction is further
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