14 results found for: “pmsf”.

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PMSF

phenylmethylsulfonyl fluoride (PMSF) is a serine protease inhibitor (serine hydrolase inactivator) commonly used in the preparation of cell lysates. PMSF does not inactivate...

Last Update: 2024-02-21T16:45:22Z Word Count : 611 Synonim PMSF

Proteinase K

65 °C, trichloroacetic acid (TCA) or the serine protease-inhibitors AEBSF, PMSF or DFP inhibit the activity. Proteinase K will not be inhibited by Guanidinium...

Last Update: 2023-11-07T16:42:20Z Word Count : 1034 Synonim Proteinase K

Diisopropyl fluorophosphate

active site Ser in serine proteases, as well as a serine protease inhibitor. PMSF and AEBSF are alternative, less toxic, but presumably also less reactive...

Last Update: 2024-02-04T04:50:24Z Word Count : 894 Synonim Diisopropyl fluorophosphate

AEBSF

plasmin, thrombin, and trypsin. The specificity is similar to the inhibitor PMSF, nevertheless AEBSF is more stable at low pH values. Typical usage is 0.1...

Last Update: 2024-01-19T12:05:30Z Word Count : 502 Synonim AEBSF

Radioimmunoprecipitation assay buffer

ingredients are optional and included as needed: Protease inhibitors (1 mM PMSF (fresh from 1 M stock in i-propanol), 1 µg/ml each leupeptin, aprotinin,...

Last Update: 2023-12-03T13:24:40Z Word Count : 322 Synonim Radioimmunoprecipitation assay buffer

Serine protease

respectively. Artificial irreversible small molecule inhibitors include AEBSF and PMSF. A family of arthropod serine peptidase inhibitors, called pacifastin, has...

Last Update: 2024-03-17T03:19:29Z Word Count : 2323 Synonim Serine protease

Protease inhibitor (biology)

(N-acetyl-L-leucyl-L-leucyl-L-argininal) alpha-2-Macroglobulin Pefabloc SC Pepstatin PMSF (phenylmethanesulfonyl fluoride) TLCK Trypsin inhibitors Kunitz domain Pacifastin...

Last Update: 2023-11-29T01:19:10Z Word Count : 1811 Synonim Protease inhibitor (biology)

European Stability Mechanism

improve the sovereign financial stability." Primary Market Support Facility (PMSF): "Bond purchase operations in the primary market could be made by ESM, in...

Last Update: 2023-11-20T21:03:26Z Word Count : 6958 Synonim European Stability Mechanism

Chymase

chymase 1, mast cell Chymase with PMSF bound PDB: 1KLT Identifiers Symbol CMA1 NCBI gene 1215 HGNC 2097 OMIM 118938 RefSeq NM_001836 UniProt P23946 Other...

Last Update: 2023-05-19T11:34:40Z Word Count : 438 Synonim Chymase

Keratinase

chromatography. The enzyme-activity was inhibited by reduced glutathione, PMSF and 2-¬Mercaptaethanol. Williams et al. (1990) continued his work on enriched...

Last Update: 2023-04-09T07:16:27Z Word Count : 748 Synonim Keratinase

22q13 deletion syndrome

doi:10.1002/ajmg.a.33253. ISSN 1552-4833. PMC 3119894. PMID 20186804. https://pmsf.org/ Oberman LM, Boccuto L, Cascio L, Sarasua S, Kaufmann WE (2015-08-27)...

Last Update: 2024-01-09T08:14:00Z Word Count : 4023 Synonim 22q13 deletion syndrome

Linda Chen

politics, she was one of the founders of the Pan-Malayan Students’ Federation (PMSF) After finishing her undergraduate degree, with a thesis on the Muslim scholar...

Last Update: 2024-03-12T13:15:24Z Word Count : 1486 Synonim Linda Chen

CMA1

v t e PDB gallery 1klt: CRYSTAL STRUCTURE OF PMSF-TREATED HUMAN CHYMASE AT 1.9 ANGSTROMS RESOLUTION 1nn6: Human Pro-Chymase 1pjp: THE 2.2 A CRYSTAL STRUCTURE...

Last Update: 2023-12-25T03:01:21Z Word Count : 939 Synonim CMA1

Mycophenolic acid acyl-glucuronide esterase

deglucuronidation of PRAG. ABHD10 has also been known to interact with: PMSF ML257 Probenecid acyl glucuronide (PRAG) Iwamura A, Fukami T, Higuchi R,...

Last Update: 2023-08-24T00:43:59Z Word Count : 740 Synonim Mycophenolic acid acyl-glucuronide esterase

Main result

PMSF

In biochemistry, phenylmethylsulfonyl fluoride (PMSF) is a serine protease inhibitor (serine hydrolase inactivator) commonly used in the preparation of cell lysates. PMSF does not inactivate all serine proteases. The effective concentration of PMSF is between 0.1 - 1 mM. The half-life is short in aqueous solutions (110 min at pH 7, 55 min at pH 7.5, and 35 min at pH 8, all at 25 °C). At 4˚C, pH 8, PMSF is almost completely degraded after 1 day. Stock solutions are usually made up in anhydrous ethanol, isopropanol, or corn oil and diluted immediately before use. PMSF reacts specifically with the active site serine residue in serine hydrolases. It does not bind to any other serine residues in the protein. This is a result of the hyperactivity of that serine residue caused by the specific environmental conditions in the enzyme's active site (catalytic triad). Because PMSF bonds covalently to the enzyme, the complex can be viewed by X-ray crystallography; it can therefore be used as a chemical label to identify an essential active site serine in an enzyme. Enzyme(active)Ser-O-H + F-SO2CH2C6H5 → EnzymeSer-O-SO2CH2C6H5 + HFSerine protease + PMSF → Irreversible enzyme-PMS complex + HFThe median lethal dose between 150–215 mg/kg (acetylcholine esterase inactivator). PMSF should be handled only inside a fume hood and while wearing gloves. DMSO is sometimes recommended as solvent for stock solutions, but should not be used as it makes intact skin permeable to PMSF.


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