20 results found for: “brdu”.

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Bromodeoxyuridine

(5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidine. BrdU is commonly used...

Last Update: 2021-03-13T21:10:39Z Word Count : 1002 Synonim Bromodeoxyuridine

Androgen

lack of external male genitalia. Neural injections of Bromodeoxyuridine (BrdU) were applied to males of both groups to test for neurogenesis. Analysis...

Last Update: 2021-03-04T18:53:23Z Word Count : 2763 Synonim Androgen

Hoechst stain

cell-permeability than H 33258. Cells can integrate BrdU in newly synthesized DNA as a substitute for thymidine. When BrdU is integrated into DNA, it is supposed that...

Last Update: 2021-01-31T11:28:26Z Word Count : 1240 Synonim Hoechst stain

Novak Djokovic

Mirjana Despot (16 October 2015). "Banjaluka dobila Novak Café na Banj brdu". PressRS.ba. Archived from the original on 9 August 2016. Retrieved 12 June...

Last Update: 2021-04-16T14:46:53Z Word Count : 25993 Synonim Novak Djokovic

Breg pri Golem Brdu

Breg pri Golem Brdu (pronounced [ˈbɾeːk pɾi ˈɡɔːlɛm ˈbəɾdu]) is a small settlement south of Golo Brdo in the Municipality of Brda in the Littoral region...

Last Update: 2017-03-14T03:53:16Z Word Count : 80 Synonim Breg pri Golem Brdu

Čukarički Stadium

Čukarički Stadium, also known as Stadion na Banovom brdu (English: Banovo Brdo Stadium) is a multi-purpose stadium in Belgrade, Serbia. It is currently...

Last Update: 2021-02-11T15:44:55Z Word Count : 96 Synonim Čukarički Stadium

Thymidine

be measured this way in lymphoproliferative disorders. Bromodeoxyuridine (BrdU) is another thymidine analog that is often used for the detection of proliferating...

Last Update: 2021-04-04T18:06:34Z Word Count : 678 Synonim Thymidine

Ayahuasca

dentate gyrus of the hippocampus noted an increase in the proliferation of BrdU positive cells in response to 100 μg of 5-MeO-DMT injected intravenously...

Last Update: 2021-04-02T03:55:22Z Word Count : 7096 Synonim Ayahuasca

HAT medium

these genes. Thus, a cell lacking TK is resistant to bromodeoxyuridine (BrdU) and a cell lacking HGPRT is resistant to 6-thioguanine (6-TG) and 8-azaguanine...

Last Update: 2019-02-17T23:51:49Z Word Count : 633 Synonim HAT medium

S phase index

the capacity of tumor cells to proliferate. It is defined as the number of BrdU-incorporating cells relative to the volume of DNA staining determined from...

Last Update: 2018-01-12T00:05:15Z Word Count : 106 Synonim S phase index

Mutation

caused by: Chemicals Hydroxylamine Base analogs (e.g., Bromodeoxyuridine (BrdU)) Alkylating agents (e.g., N-ethyl-N-nitrosourea (ENU). These agents can...

Last Update: 2021-04-15T09:07:47Z Word Count : 11067 Synonim Mutation

Republika Srpska

November 2017. Retrieved 29 August 2011. "Pobjednik Grmečke koride na Popovića brdu prošlogodišnji - bik Medonja". Sve o Srpskoj (in Serbian). 8 August 2020...

Last Update: 2021-04-15T18:57:03Z Word Count : 8959 Synonim Republika Srpska

Immunohistochemistry

and predictive biomarkers. Examples of some commonly used markers include: BrdU: used to identify replicating cells. Used to identify tumors as well as in...

Last Update: 2021-01-06T12:59:31Z Word Count : 3180 Synonim Immunohistochemistry

Local lymph node assay

specific for BrdU). The test material is applied to the ears of mice. Optionally, a tracer substance such as 3H-Methyl-thymidine or BrdU is injected intraperitoneally...

Last Update: 2018-02-16T15:37:28Z Word Count : 445 Synonim Local lymph node assay

Single cell sequencing

requires dividing cells for strand-specific labelling using bromodeoxyuridine (BrdU), and the method does not detect genetic variants smaller than 200kb in size...

Last Update: 2021-04-14T18:36:50Z Word Count : 7725 Synonim Single cell sequencing

TUNEL assay

incorporation of BrdUTP by TdT followed by immunocytochemical detection of BrdU. Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z (1993). "Presence...

Last Update: 2020-12-12T01:03:52Z Word Count : 747 Synonim TUNEL assay

Mixed lymphocyte reaction

incorporating radioactive [3H] labelled thymidine or 5-bromo-2’-deoxyuridine (BrdU), into the mixed cell suspension. This cellular response to the histocompatibility...

Last Update: 2020-06-04T07:39:33Z Word Count : 721 Synonim Mixed lymphocyte reaction

Doublecortin

response to exercise, and that increase occurs in parallel with increased BrdU labeling, which is currently a "gold standard" in measuring neurogenesis...

Last Update: 2020-12-11T16:33:38Z Word Count : 1840 Synonim Doublecortin

Immortal DNA strand hypothesis

strands with a DNA label such as tritiated thymidine or bromodeoxyuridine (BrdU). These types of DNA labels will incorporate into the newly synthesized DNA...

Last Update: 2020-12-13T13:53:38Z Word Count : 2233 Synonim Immortal DNA strand hypothesis

Cell cycle analysis

DNA content and of incorporation of DNA precursor 5-bromo-2'-deoxyuridine (BrdU) by flow cytometry is an especially useful assay, that has been widely used...

Last Update: 2021-01-02T20:38:14Z Word Count : 1527 Synonim Cell cycle analysis

Main result

Bromodeoxyuridine

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidine. BrdU is commonly used to study cell proliferation in living tissues and has been studied as a radiosensitizer and diagnostic tool in people with cancer.During S phase of the cell cycle (when DNA replication occurs), BrdU can be incorporated in place of thymidine in newly synthesized DNA molecules of dividing cells. Cells that have recently performed DNA replication or DNA repair can be detected with antibodies specific for BrdU using techniques such as immunohistochemistry or immunofluorescence. BrdU-labelled cells in humans can be detected up to two years after BrdU infusion.Because BrdU can replace thymidine during DNA replication, it can cause mutations, and its use is therefore potentially a health hazard. However, because it is neither radioactive nor myelotoxic at labeling concentrations, it is widely preferred for in vivo studies of cancer cell proliferation. However, at radiosensitizing concentrations, BrdU becomes myelosuppressive, thus limiting its use for radiosensitizing.BrdU differs from thymidine in that BrdU substitutes a bromine atom for thymidine's CH3 group. The Br substitution can be used in X-ray diffraction experiments in crystals containing either DNA or RNA. The Br atom acts as an anomalous scatterer and its larger size will affect the crystal's X-ray diffraction enough to detect isomorphous differences as well.Bromodeoxyuridine releases gene silencing caused by DNA methylation.BrdU can also be used to identify microorganisms that respond to specific carbon substrates in aquatic and soil environments. A carbon substrate added to incubations of environmental samples will cause the growth of microorganisms that can utilize that substrate. These microorganisms will then incorporate BrdU into their DNA as they grow. Community DNA can then be isolated and BrdU-labeled DNA purified using an immunocapture technique. Subsequent sequencing of the labeled DNA can then be used to identify the microbial taxa that participated in the degradation of the added carbon source. However, it is not certain whether all microbes present in an environmental sample can incorporate BrdU into their biomass during de novo DNA synthesis. Therefore, a group of microorganisms may respond to a carbon source but go undetected using this technique. Additionally, this technique is biased towards identifying microorganisms with A- and T-rich genomes.


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