Fluorescence anisotropy

Fluorescence anisotropy or fluorescence polarization is the phenomenon where the light emitted by a fluorophore has unequal intensities along different axes of polarization. Early pioneers in the field include Aleksander Jablonski, Gregorio Weber,[1] and Andreas Albrecht.[2] The principles of fluorescence polarization and some applications of the method are presented in Lakowicz's book.[3]

  1. ^ Weber, G., 1953. Rotational Brownian motion and polarization of the fluorescence of solutions. Adv. Protein Chem. 8:415-459
  2. ^ Albrecht, A., 1961. Polarizations and assignments of transitions: the method of photoselection. J. Mol. Spectrosc. 6:84-108.
  3. ^ Lakowicz, J.R., 2006. Principles of Fluorescence Spectroscopy (3rd ed., Springer. Chapter 10-12 deal with fluorescence polarization spectroscopy.)